goat anti dpp iv  (R&D Systems)

Journal: OncoTargets and Therapy

Article Title: Prognostic significance of the combined expression of neutral endopeptidase and dipeptidyl peptidase IV in intrahepatic cholangiocarcinoma patients after surgery resection

doi: 10.2147/ott.s57355

Figure Lengend Snippet: Figure 1 Immunohistochemical staining for NEP and DPP IV in IHCC and noncancerous liver tissues. Notes: Original magnification ×200. (A and B) NEP and DPP IV strongly positive expression was found in cell membrane and cytoplasm at various levels in IHCC tissues. (C and D) NEP and DPP IV weakly positive expression was found in cell membrane and cytoplasm at various levels in IHCC tissues. (E and F) NEP and DPP IV negative expression in noncancerous liver tissues. Abbreviations: DPP IV, dipeptidyl peptidase IV; IHCC, intrahepatic cholangio carcinoma; NEP, neutral endopeptidase.

Article Snippet: Subsequently, the slides were incubated overnight with the primary antibodies against anti-NEP mouse monoclonal antibody (#sc-46656; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) or anti-DPP IV goat polyclonal antibody (#AF1180, R&D Systems, Minneapolis, MN, USA) at 4°C.

Techniques: Immunohistochemical staining, Staining, Expressing, Membrane

Journal: OncoTargets and Therapy

Article Title: Prognostic significance of the combined expression of neutral endopeptidase and dipeptidyl peptidase IV in intrahepatic cholangiocarcinoma patients after surgery resection

doi: 10.2147/ott.s57355

Figure Lengend Snippet: Figure 2 Overall survival and recurrence-free survival curves for two groups defined by low and high expression of NEP or DPP IV, and for four groups defined by combined expression of NEP and DPP IV, of patients with IHCC. Notes: Kaplan–Meier analysis showed IHCC patients with high NEP expression, high DPP IV expression, and combined overexpression of NEP and DPP IV proteins all had poorer overall survival and early recurrence after surgery. (A and B) low and high expression of NEP. (C and D) low and high expression DPP IV. (E and F) combined expression of NEP and DPP IV. Abbreviations: DPP IV, dipeptidyl peptidase IV; IHCC, intrahepatic cholangiocarcinoma; NEP, neutral endopeptidase.

Article Snippet: Subsequently, the slides were incubated overnight with the primary antibodies against anti-NEP mouse monoclonal antibody (#sc-46656; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) or anti-DPP IV goat polyclonal antibody (#AF1180, R&D Systems, Minneapolis, MN, USA) at 4°C.

Techniques: Expressing, Over Expression

Journal: Diabetes & vascular disease research

Article Title: Dipeptidyl peptidase-IV is a potential molecular biomarker in diabetic kidney disease.

doi: 10.1177/1479164111434318

Figure Lengend Snippet: Figure 4. Western blotting with anti-dipeptidyl peptidase-IV (DPP IV) antibody. 1. Urine immunoprecipitated with anti-DPP IV antibody. 2. Urine immunoprecipitated with AD-1 antibody treated with Triton X-100. 3. Urine immunoprecipitated with AD-1 antibody. 4. Serum immunoprecipitated with anti-DPP IV antibody. Figure 5. Electron micrograph of urinary microvesicles.

Article Snippet: Membranes were stained with Ponceau S to check the transferring efficiency, briefly washed with PBST, blocked in PBST/5% low-fat milk for 30 min, and probed with a goat anti-human DPP IV antibody (R&D systems, AF1180) overnight in PBST/1% low-fat milk.

Techniques: Western Blot, Immunoprecipitation